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negative control pcmv3 c ha ncv  (Sino Biological)


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    Structured Review

    Sino Biological negative control pcmv3 c ha ncv
    Negative Control Pcmv3 C Ha Ncv, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv3/pCMV3-C-HA+Negative+Control+Vector/pm42043249-86-7-13
    Average 94 stars, based on 50 article reviews
    negative control pcmv3 c ha ncv - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Over Expression:

    Article Title: Occludin modulates HIV and ischaemic stroke response via mitochondrial antiviral signalling pathway.
    Article Snippet: Trilencer-27-universal scrambled 23 (SCR) silencer siRNA duplex (OriGene, Cat# SR30004) was used as a negative control. .. For ocln 24 overexpression, cells were transfected with the PCMV3-OCLN plasmid (Sino Biological, Wayne, 25 PA, Cat# HG15134-UT) or PCMV3 (Sino Biological, Cat# CV011) as a negative control. .. A total 26 of 0.5 μg siRNA per 106 of cells and 1 (+) or 1.5 μg DNA (++) per 106 of cells were transfected 27 by Amaxa Nucleofector Technology using the Basic Nucleofector Kit (Lonza, Switzerland, EU, 28 AC CE PT ED M AN US CR IP T Downloaded from https://academ ic.oup.com /brain/advance-article/doi/10.1093/brain/aw af262/8202977 by guest on 17 July 2025 Cat# VPI‐1001).

    Article Title: Occludin modulates HIV and ischaemic stroke response via the mitochondrial antiviral signalling pathway
    Article Snippet: Trilencer-27-universal scrambled (SCR) silencer siRNA duplex (OriGene, Cat. No. SR30004) was used as a negative control. .. For Ocln overexpression, cells were transfected with the PCMV3-OCLN plasmid (Sino Biological, Cat. No. HG15134-UT) or PCMV3 (Sino Biological, Cat. No. CV011) as a negative control. .. A total of 0.5 μg siRNA per 10 6 of cells and 1 (+) or 1.5 μg DNA (++) per 10 6 of cells were transfected by Amaxa Nucleofector Technology using the Basic Nucleofector Kit (Lonza, Cat. No. VPI-1001).

    Transfection:

    Article Title: Occludin modulates HIV and ischaemic stroke response via mitochondrial antiviral signalling pathway.
    Article Snippet: Trilencer-27-universal scrambled 23 (SCR) silencer siRNA duplex (OriGene, Cat# SR30004) was used as a negative control. .. For ocln 24 overexpression, cells were transfected with the PCMV3-OCLN plasmid (Sino Biological, Wayne, 25 PA, Cat# HG15134-UT) or PCMV3 (Sino Biological, Cat# CV011) as a negative control. .. A total 26 of 0.5 μg siRNA per 106 of cells and 1 (+) or 1.5 μg DNA (++) per 106 of cells were transfected 27 by Amaxa Nucleofector Technology using the Basic Nucleofector Kit (Lonza, Switzerland, EU, 28 AC CE PT ED M AN US CR IP T Downloaded from https://academ ic.oup.com /brain/advance-article/doi/10.1093/brain/aw af262/8202977 by guest on 17 July 2025 Cat# VPI‐1001).

    Article Title: An increase of lysosomes through EGF-triggered endocytosis attenuated zinc-mediated lysosomal membrane permeabilization and neuronal cell death
    Article Snippet: .. Near-pure cortical neuronal cells were transfected with either the pCMV3-untagged negative control vector (Sino Biological, Beijing, China) or the LAMP-1-GFP plasmid using program CU110 of the Amaxa 4D Nucleofector system (Lonza, Swiss). .. HEK cells were transfected with 250 ng/ml pCMV3-untagged negative control vector (Sino Biological, Beijing, China), LAMP-1-RFP plasmid or EGFR-GFP plasmid for 4 hours using Lipofectamine 2000 (Invitrogen).

    Article Title: An increase of lysosomes through EGF-triggered endocytosis attenuated zinc-mediated lysosomal membrane permeabilization and neuronal cell death
    Article Snippet: Near-pure cortical neuronal cells were transfected with either the pCMV3-untagged negative control vector (Sino Biological, Beijing, China) or the LAMP-1-GFP plasmid using program CU110 of the Amaxa 4D Nucleofector system (Lonza, Swiss). .. HEK cells were transfected with 250 ng/ml pCMV3-untagged negative control vector (Sino Biological, Beijing, China), LAMP-1-RFP plasmid or EGFR-GFP plasmid for 4 hours using Lipofectamine 2000 (Invitrogen). ..

    Article Title: FSP1 reduces exogenous coenzyme Q10 and inhibits ferroptosis to alleviate intestinal ischemia-reperfusion injury.
    Article Snippet: Two micrograms of pCMV3FSP1 (Sino Biological, MG52065-UT) or pCMV3-untagged negative control vector plasmid (Sino Biological, CV011) diluted in OptiMEM was transfected into Caco-2 cells using transfection reagent. .. Two micrograms of pCMV3FSP1 (Sino Biological, MG52065-UT) or pCMV3-untagged negative control vector plasmid (Sino Biological, CV011) diluted in OptiMEM was transfected into Caco-2 cells using transfection reagent. ..

    Article Title: Loss of ELF2 drives topotecan resistance in retinoblastoma revealed by genome-wide CRISPR-Cas9 screening
    Article Snippet: .. The human ELF2 ORF clone expression plasmid (HG12537-UT) and pCMV3-untagged negative control vector (CV011; Sino Biological, Beijing, China) were transfected into WERI-Rb1 and Y79 cells separately using LipofectamineTM 3000 transfection reagent (L3000075; Thermo Fisher Scientific, US). ..

    Article Title: Loss of ELF2 drives topotecan resistance in retinoblastoma revealed by genome-wide CRISPR-Cas9 screening.
    Article Snippet: .. The human ELF2 ORF clone expression plasmid (HG12537-UT) and pCMV3-untagged negative control vector (CV011; Sino Biological, Beijing, China) were transfected into WERI-Rb1 and Y79 cells separately using LipofectamineTM 3000 transfection reagent (L3000075; Thermo Fisher Scientific, US). ..

    Article Title: Occludin modulates HIV and ischaemic stroke response via the mitochondrial antiviral signalling pathway
    Article Snippet: Trilencer-27-universal scrambled (SCR) silencer siRNA duplex (OriGene, Cat. No. SR30004) was used as a negative control. .. For Ocln overexpression, cells were transfected with the PCMV3-OCLN plasmid (Sino Biological, Cat. No. HG15134-UT) or PCMV3 (Sino Biological, Cat. No. CV011) as a negative control. .. A total of 0.5 μg siRNA per 10 6 of cells and 1 (+) or 1.5 μg DNA (++) per 10 6 of cells were transfected by Amaxa Nucleofector Technology using the Basic Nucleofector Kit (Lonza, Cat. No. VPI-1001).

    Plasmid Preparation:

    Article Title: Occludin modulates HIV and ischaemic stroke response via mitochondrial antiviral signalling pathway.
    Article Snippet: Trilencer-27-universal scrambled 23 (SCR) silencer siRNA duplex (OriGene, Cat# SR30004) was used as a negative control. .. For ocln 24 overexpression, cells were transfected with the PCMV3-OCLN plasmid (Sino Biological, Wayne, 25 PA, Cat# HG15134-UT) or PCMV3 (Sino Biological, Cat# CV011) as a negative control. .. A total 26 of 0.5 μg siRNA per 106 of cells and 1 (+) or 1.5 μg DNA (++) per 106 of cells were transfected 27 by Amaxa Nucleofector Technology using the Basic Nucleofector Kit (Lonza, Switzerland, EU, 28 AC CE PT ED M AN US CR IP T Downloaded from https://academ ic.oup.com /brain/advance-article/doi/10.1093/brain/aw af262/8202977 by guest on 17 July 2025 Cat# VPI‐1001).

    Article Title: An increase of lysosomes through EGF-triggered endocytosis attenuated zinc-mediated lysosomal membrane permeabilization and neuronal cell death
    Article Snippet: .. Near-pure cortical neuronal cells were transfected with either the pCMV3-untagged negative control vector (Sino Biological, Beijing, China) or the LAMP-1-GFP plasmid using program CU110 of the Amaxa 4D Nucleofector system (Lonza, Swiss). .. HEK cells were transfected with 250 ng/ml pCMV3-untagged negative control vector (Sino Biological, Beijing, China), LAMP-1-RFP plasmid or EGFR-GFP plasmid for 4 hours using Lipofectamine 2000 (Invitrogen).

    Article Title: An increase of lysosomes through EGF-triggered endocytosis attenuated zinc-mediated lysosomal membrane permeabilization and neuronal cell death
    Article Snippet: Near-pure cortical neuronal cells were transfected with either the pCMV3-untagged negative control vector (Sino Biological, Beijing, China) or the LAMP-1-GFP plasmid using program CU110 of the Amaxa 4D Nucleofector system (Lonza, Swiss). .. HEK cells were transfected with 250 ng/ml pCMV3-untagged negative control vector (Sino Biological, Beijing, China), LAMP-1-RFP plasmid or EGFR-GFP plasmid for 4 hours using Lipofectamine 2000 (Invitrogen). ..

    Article Title: FSP1 reduces exogenous coenzyme Q10 and inhibits ferroptosis to alleviate intestinal ischemia-reperfusion injury.
    Article Snippet: Two micrograms of pCMV3FSP1 (Sino Biological, MG52065-UT) or pCMV3-untagged negative control vector plasmid (Sino Biological, CV011) diluted in OptiMEM was transfected into Caco-2 cells using transfection reagent. .. Two micrograms of pCMV3FSP1 (Sino Biological, MG52065-UT) or pCMV3-untagged negative control vector plasmid (Sino Biological, CV011) diluted in OptiMEM was transfected into Caco-2 cells using transfection reagent. ..

    Article Title: Loss of ELF2 drives topotecan resistance in retinoblastoma revealed by genome-wide CRISPR-Cas9 screening
    Article Snippet: .. The human ELF2 ORF clone expression plasmid (HG12537-UT) and pCMV3-untagged negative control vector (CV011; Sino Biological, Beijing, China) were transfected into WERI-Rb1 and Y79 cells separately using LipofectamineTM 3000 transfection reagent (L3000075; Thermo Fisher Scientific, US). ..

    Article Title: Loss of ELF2 drives topotecan resistance in retinoblastoma revealed by genome-wide CRISPR-Cas9 screening.
    Article Snippet: .. The human ELF2 ORF clone expression plasmid (HG12537-UT) and pCMV3-untagged negative control vector (CV011; Sino Biological, Beijing, China) were transfected into WERI-Rb1 and Y79 cells separately using LipofectamineTM 3000 transfection reagent (L3000075; Thermo Fisher Scientific, US). ..

    Article Title: Occludin modulates HIV and ischaemic stroke response via the mitochondrial antiviral signalling pathway
    Article Snippet: Trilencer-27-universal scrambled (SCR) silencer siRNA duplex (OriGene, Cat. No. SR30004) was used as a negative control. .. For Ocln overexpression, cells were transfected with the PCMV3-OCLN plasmid (Sino Biological, Cat. No. HG15134-UT) or PCMV3 (Sino Biological, Cat. No. CV011) as a negative control. .. A total of 0.5 μg siRNA per 10 6 of cells and 1 (+) or 1.5 μg DNA (++) per 10 6 of cells were transfected by Amaxa Nucleofector Technology using the Basic Nucleofector Kit (Lonza, Cat. No. VPI-1001).

    Negative Control:

    Article Title: Occludin modulates HIV and ischaemic stroke response via mitochondrial antiviral signalling pathway.
    Article Snippet: Trilencer-27-universal scrambled 23 (SCR) silencer siRNA duplex (OriGene, Cat# SR30004) was used as a negative control. .. For ocln 24 overexpression, cells were transfected with the PCMV3-OCLN plasmid (Sino Biological, Wayne, 25 PA, Cat# HG15134-UT) or PCMV3 (Sino Biological, Cat# CV011) as a negative control. .. A total 26 of 0.5 μg siRNA per 106 of cells and 1 (+) or 1.5 μg DNA (++) per 106 of cells were transfected 27 by Amaxa Nucleofector Technology using the Basic Nucleofector Kit (Lonza, Switzerland, EU, 28 AC CE PT ED M AN US CR IP T Downloaded from https://academ ic.oup.com /brain/advance-article/doi/10.1093/brain/aw af262/8202977 by guest on 17 July 2025 Cat# VPI‐1001).

    Article Title: An increase of lysosomes through EGF-triggered endocytosis attenuated zinc-mediated lysosomal membrane permeabilization and neuronal cell death
    Article Snippet: .. Near-pure cortical neuronal cells were transfected with either the pCMV3-untagged negative control vector (Sino Biological, Beijing, China) or the LAMP-1-GFP plasmid using program CU110 of the Amaxa 4D Nucleofector system (Lonza, Swiss). .. HEK cells were transfected with 250 ng/ml pCMV3-untagged negative control vector (Sino Biological, Beijing, China), LAMP-1-RFP plasmid or EGFR-GFP plasmid for 4 hours using Lipofectamine 2000 (Invitrogen).

    Article Title: An increase of lysosomes through EGF-triggered endocytosis attenuated zinc-mediated lysosomal membrane permeabilization and neuronal cell death
    Article Snippet: Near-pure cortical neuronal cells were transfected with either the pCMV3-untagged negative control vector (Sino Biological, Beijing, China) or the LAMP-1-GFP plasmid using program CU110 of the Amaxa 4D Nucleofector system (Lonza, Swiss). .. HEK cells were transfected with 250 ng/ml pCMV3-untagged negative control vector (Sino Biological, Beijing, China), LAMP-1-RFP plasmid or EGFR-GFP plasmid for 4 hours using Lipofectamine 2000 (Invitrogen). ..

    Article Title: FSP1 reduces exogenous coenzyme Q10 and inhibits ferroptosis to alleviate intestinal ischemia-reperfusion injury.
    Article Snippet: Two micrograms of pCMV3FSP1 (Sino Biological, MG52065-UT) or pCMV3-untagged negative control vector plasmid (Sino Biological, CV011) diluted in OptiMEM was transfected into Caco-2 cells using transfection reagent. .. Two micrograms of pCMV3FSP1 (Sino Biological, MG52065-UT) or pCMV3-untagged negative control vector plasmid (Sino Biological, CV011) diluted in OptiMEM was transfected into Caco-2 cells using transfection reagent. ..

    Article Title: Loss of ELF2 drives topotecan resistance in retinoblastoma revealed by genome-wide CRISPR-Cas9 screening
    Article Snippet: .. The human ELF2 ORF clone expression plasmid (HG12537-UT) and pCMV3-untagged negative control vector (CV011; Sino Biological, Beijing, China) were transfected into WERI-Rb1 and Y79 cells separately using LipofectamineTM 3000 transfection reagent (L3000075; Thermo Fisher Scientific, US). ..

    Article Title: Loss of ELF2 drives topotecan resistance in retinoblastoma revealed by genome-wide CRISPR-Cas9 screening.
    Article Snippet: .. The human ELF2 ORF clone expression plasmid (HG12537-UT) and pCMV3-untagged negative control vector (CV011; Sino Biological, Beijing, China) were transfected into WERI-Rb1 and Y79 cells separately using LipofectamineTM 3000 transfection reagent (L3000075; Thermo Fisher Scientific, US). ..

    Article Title: Occludin modulates HIV and ischaemic stroke response via the mitochondrial antiviral signalling pathway
    Article Snippet: Trilencer-27-universal scrambled (SCR) silencer siRNA duplex (OriGene, Cat. No. SR30004) was used as a negative control. .. For Ocln overexpression, cells were transfected with the PCMV3-OCLN plasmid (Sino Biological, Cat. No. HG15134-UT) or PCMV3 (Sino Biological, Cat. No. CV011) as a negative control. .. A total of 0.5 μg siRNA per 10 6 of cells and 1 (+) or 1.5 μg DNA (++) per 10 6 of cells were transfected by Amaxa Nucleofector Technology using the Basic Nucleofector Kit (Lonza, Cat. No. VPI-1001).

    Expressing:

    Article Title: Loss of ELF2 drives topotecan resistance in retinoblastoma revealed by genome-wide CRISPR-Cas9 screening
    Article Snippet: .. The human ELF2 ORF clone expression plasmid (HG12537-UT) and pCMV3-untagged negative control vector (CV011; Sino Biological, Beijing, China) were transfected into WERI-Rb1 and Y79 cells separately using LipofectamineTM 3000 transfection reagent (L3000075; Thermo Fisher Scientific, US). ..

    Article Title: Loss of ELF2 drives topotecan resistance in retinoblastoma revealed by genome-wide CRISPR-Cas9 screening.
    Article Snippet: .. The human ELF2 ORF clone expression plasmid (HG12537-UT) and pCMV3-untagged negative control vector (CV011; Sino Biological, Beijing, China) were transfected into WERI-Rb1 and Y79 cells separately using LipofectamineTM 3000 transfection reagent (L3000075; Thermo Fisher Scientific, US). ..



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